Enhanced Electrocatalytic Properties of Co3O4 Nanocrystals Derived from Hydrolyzed Polyethyleneimines in Water/Ethanol Solvents for Electrochemical Detection of Cholesterol

The present study describes the effect of hydrolysis polyethyleneimines in water/ethanol mixture on morphology cobalt oxide (Co3O4), used as main sensor component. structure generated Co3O4 nanocrystals is consistent with a well-defined cubic phase crystallography, having only and oxygen elements. Developing simple, low-cost, sensitive, selective cholesterol biosensors essential for accurate monitoring to avoid cardiovascular diseases. These exhibit large surfaces suitable facile high loading oxidase enzyme through physical adsorption method. Then, fabricated oxidase/ composite was implemented potentiometric detection 10 mM phosphate buffer pH 7.3. Importantly, presented biosensor revealed wide linear range 0.005 3.0 limit (LOD) 0.001 mM. Additionally, sensitivity estimated around 60 mVdec?1. selectivity, stability, reproducibility, repeatability were also observed satisfactory. dynamic response proposed method demonstrated fast time less than 1 s. Furthermore, successive addition confirmed remarkably stable towards various concentrations. Thus, developed nanocomposite may be an efficient alternative monitor low concentrations form real samples.